Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies.
Identifieur interne : 004796 ( Main/Exploration ); précédent : 004795; suivant : 004797Immunological detection of severe acute respiratory syndrome coronavirus by monoclonal antibodies.
Auteurs : Kazuo Ohnishi [Japon] ; Masahiro Sakaguchi ; Tomohiro Kaji ; Kiyoko Akagawa ; Tadayoshi Taniyama ; Masataka Kasai ; Yasuko Tsunetsugu-Yokota ; Masamichi Oshima ; Kiichi Yamamoto ; Naomi Takasuka ; Shu-Ichi Hashimoto ; Manabu Ato ; Hideki Fujii ; Yoshimasa Takahashi ; Shigeru Morikawa ; Koji Ishii ; Tetsutaro Sata ; Hirotaka Takagi ; Shigeyuki Itamura ; Takato Odagiri ; Tatsuo Miyamura ; Ichiro Kurane ; Masato Tashiro ; Takeshi Kurata ; Hiroshi Yoshikura ; Toshitada TakemoriSource :
- Japanese journal of infectious diseases [ 1344-6304 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Animaux, Anticorps monoclonaux, Cellules Vero, Femelle, Humains, Immunohistochimie, Sensibilité et spécificité, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- MESH :
- chemical : Antibodies, Monoclonal.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- Animals, Chlorocebus aethiops, Female, Humans, Immunohistochemistry, Mice, Mice, Inbred BALB C, Sensitivity and Specificity, Vero Cells.
Abstract
In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.
PubMed: 15858286
Affiliations:
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first="Masato" last="Tashiro">Masato Tashiro</name></author><author><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name></author><author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name></author><author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15858286</idno><idno type="pmid">15858286</idno><idno type="wicri:Area/PubMed/Corpus">002765</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002765</idno><idno type="wicri:Area/PubMed/Curation">002765</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002765</idno><idno 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Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Tokyo 162-8640</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name></author><author><name sortKey="Kaji, Tomohiro" sort="Kaji, Tomohiro" uniqKey="Kaji T" first="Tomohiro" last="Kaji">Tomohiro Kaji</name></author><author><name sortKey="Akagawa, Kiyoko" sort="Akagawa, Kiyoko" uniqKey="Akagawa K" first="Kiyoko" last="Akagawa">Kiyoko Akagawa</name></author><author><name sortKey="Taniyama, Tadayoshi" sort="Taniyama, Tadayoshi" uniqKey="Taniyama T" first="Tadayoshi" last="Taniyama">Tadayoshi Taniyama</name></author><author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name></author><author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name></author><author><name sortKey="Oshima, Masamichi" sort="Oshima, Masamichi" uniqKey="Oshima M" first="Masamichi" last="Oshima">Masamichi Oshima</name></author><author><name sortKey="Yamamoto, Kiichi" sort="Yamamoto, Kiichi" uniqKey="Yamamoto K" first="Kiichi" last="Yamamoto">Kiichi Yamamoto</name></author><author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name></author><author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name></author><author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name></author><author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name></author><author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author><author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name></author><author><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name></author><author><name sortKey="Takagi, Hirotaka" sort="Takagi, Hirotaka" uniqKey="Takagi H" first="Hirotaka" last="Takagi">Hirotaka Takagi</name></author><author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name></author><author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name></author><author><name sortKey="Miyamura, Tatsuo" sort="Miyamura, Tatsuo" uniqKey="Miyamura T" first="Tatsuo" last="Miyamura">Tatsuo Miyamura</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name></author><author><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name></author><author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name></author><author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name></author></analytic><series><title level="j">Japanese journal of infectious diseases</title><idno type="ISSN">1344-6304</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cellules Vero</term><term>Femelle</term><term>Humains</term><term>Immunohistochimie</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Female</term><term>Humans</term><term>Immunohistochemistry</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cellules Vero</term><term>Femelle</term><term>Humains</term><term>Immunohistochimie</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to establish immunological detection methods for severe acute respiratory syndrome coronavirus (SARS-CoV), we established monoclonal antibodies directed against structural components of the virus. B cell hybridomas were generated from mice that were hyper-immunized with inactivated SARS-CoV virion. By screening 2,880 generated hybridomas, we established three hybridoma clones that secreted antibodies specific for nucleocapsid protein (N) and 27 clones that secreted antibodies specific for spike protein (S). Among these, four S-protein specific antibodies had in vitro neutralization activity against SARS-CoV infection. These monoclonal antibodies enabled the immunological detection of SARS-CoV by immunofluorescence staining, Western blot or immunohistology. Furthermore, a combination of monoclonal antibodies with different specificities allowed the establishment of a highly sensitive antigen-capture sandwich ELISA system. These monoclonal antibodies would be a useful tool for rapid and specific diagnosis of SARS and also for possible antibody-based treatment of the disease.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Akagawa, Kiyoko" sort="Akagawa, Kiyoko" uniqKey="Akagawa K" first="Kiyoko" last="Akagawa">Kiyoko Akagawa</name><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name><name sortKey="Kaji, Tomohiro" sort="Kaji, Tomohiro" uniqKey="Kaji T" first="Tomohiro" last="Kaji">Tomohiro Kaji</name><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><name sortKey="Kurata, Takeshi" sort="Kurata, Takeshi" uniqKey="Kurata T" first="Takeshi" last="Kurata">Takeshi Kurata</name><name sortKey="Miyamura, Tatsuo" sort="Miyamura, Tatsuo" uniqKey="Miyamura T" first="Tatsuo" last="Miyamura">Tatsuo Miyamura</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name><name sortKey="Oshima, Masamichi" sort="Oshima, Masamichi" uniqKey="Oshima M" first="Masamichi" last="Oshima">Masamichi Oshima</name><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name><name sortKey="Sata, Tetsutaro" sort="Sata, Tetsutaro" uniqKey="Sata T" first="Tetsutaro" last="Sata">Tetsutaro Sata</name><name sortKey="Takagi, Hirotaka" sort="Takagi, Hirotaka" uniqKey="Takagi H" first="Hirotaka" last="Takagi">Hirotaka Takagi</name><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name><name sortKey="Taniyama, Tadayoshi" sort="Taniyama, Tadayoshi" uniqKey="Taniyama T" first="Tadayoshi" last="Taniyama">Tadayoshi Taniyama</name><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name><name sortKey="Yamamoto, Kiichi" sort="Yamamoto, Kiichi" uniqKey="Yamamoto K" first="Kiichi" last="Yamamoto">Kiichi Yamamoto</name><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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